Pen/Strep-PreMix for elimination and prevention of bacterial contamination in cell culture.
cell culture quality
For elimination and prevention of bacterial contamination in cell culture.
ADR 6.1 III / WGK 1
Antibiotic compound of penicillin, streptomycin and stabilising sodium
chloride for application in cell culture. The premixed powder is extremely
stable during storage at room temperature and, due to the excellent
solubility of the reagents in pyrogen-free water (e.g. Art. No. 3255.1),
it can be mixed quickly to a 100 x stock solution.
Penicillin inhibits cell wall biosynthesis in Gram-positive bacteria.
Streptomycin produces the antibiotic effect in Gram-negative
bacteria through inhibition of the translation.
The dissolved antibiotics are stable for approx. 3 days at 37 °C.
The final concentration of the 100 x stock solution is:
Penicillin: 10.000 I.U. / ml
Streptomycin: 10 mg / ml
Sodium chloride: 0.9 %
Endotoxin content per vial: <=210 l.U. complies <=4.2 l.U. / ml
Application: The pack contains 2 vials with premixed powder for 2 times 50 ml 100 x stock solution. 50 ml of the 100 x stock solution can be obtained directly by adding 49.2 ml pyrogen-free water (Art. No. 3355).
We recommend application in a 1:100 dilution.
The stock solution mix (100 ml) is sufficient for 10 l medium.
Storage of stock solution: in 1-10 ml aliquots at -20 °C.
Comes with original seal.
Penicillin G sodium salt 312,5 mg/vial
Streptomycin 0,5 g/vial
Sodium chloride 0,45 g/vial
Endotoxins <=210 I.U./vial
pH-value (stock solution in water) 5,5±0,2